Dna And Rna Nuclease Place Of Origin: China

Benzonase is a kind of endonuclease originally derived from Serratia marcescens. The enzyme is produced and manufactured from genetically modified E. coli strain, containing the expression production plasmid. Structurally, the protein is a dimer of identical 245 amino acid, ~30 kDa subunits with two essential disulfide bonds. This promiscuous endonuclease attacks and degrades all forms of DNA and RNA (single stranded, double stranded, linear and circular) and is effective over a wide range of operating conditions. This product completely digests nucleic acids to 5'-monophosphate terminated oligonucleotides 2 to 5 bases in length. Although the nuclease is capable of cleavage at nearly all positions along a nucleic acid chain. it prefers GC-rich regions in dsDNA while avoiding d(A)/d(T)-tracts. Benzonase is now available from Hangzhou Junfeng Bioengineering Co., Ltd, Purity > 99%. The preparation possesses exceptionally high specific activities and are supplied free from measurable protease activities and viral contaminants. Benzonase is ideal for a wide variety of applications where complete digestion of nucleic acids is desirable.
Unit definition
One unit of Benzonase Nuclease is defined as the amount of enzyme that causes a A A A260 of 1.0 in 30 min, which corresponds to complete digestion of 37 A Aug of DNA. Standard reaction conditions are 1 mg/ml sonicated DNA substrate in 50 mM Tris-HCl pH 8.0, 0.1 mg/ml BSA, 1 mM MgCl2, incubated at 37A A C; measured as perchloric acid-soluble digestion product.
Storage
Benzonase Nuclease is supplied in 50% glycerol containing 50 mM Tris-HCl pH 8.0,20 mM NaCl and 2 mM MgCl2. The enzyme preparation is stable for 2 years when stored at 20A C.
DO NOT store at 70A C as freezing Benzonase Nuclease results in loss of activity.