Pfu Dna Polymerase

This Recombinant PFU DNA Polymerase is a thermostable enzyme of approximately 90kDa isolated. The enzyme replicates DNA at 75oC, catalyzing the polymerization of nucleotides into duplex DNA in the 5A'-3A' direction in the presence of magnesium. Recombinant PFU DNA Polymerase also possesses 3A'-5A' exonuclease (proofreading) activity. Base misinsertions that may occur during polymerization are rapidly excised by the proofreading activity of the polymerase. Consequently, Pfu DNA Polymerase is recommended for use in PCR and primer extension reactions that require high-fidelity synthesis. Pfu DNA Polymerase-generated PCR fragments are blunt-ended.
Recombinant PFU DNA Polymerase exhibits the lowest error rate of any thermostable DNA polymerase studied (1-3). For routine PCR, where simple detection of an amplification product or estimation of the product's size is important, Taq DNA polymerase is the obvious enzyme to choose. However, when the amplified product is to be cloned, expressed or used in mutagenesis studies, Pfu DNA Polymerase is a much better enzyme of choice for PCR. Pfu DNA Polymerase is also used in blends with Taq DNA polymerase, or amino-terminally truncated versions of Taq DNA polymerase, to amplify longer stretches of DNA in PCR with greater accuracy than Taq DNA polymerase alone.