Enterokinase Recombinant

Recombinant Enterokinase is a highly purified preparation of the light chain of bovine enterokinase with high specificity and recognizes the identical cleavage site as the native enzyme. The product cleaves fusion proteins with sequence (Asp-Asp-Asp-Asp-Lys ) at the C-terminal of Lysine. The enzyme is produced in recombinant E.coli and purified to high homogeneity with specificity of 30,000U/mg. It can work at various different conditions, such as 4-45 degree, pH4.5-9.5, even in some detergents or denature reagents.
Purity: More than 95% by SDS-PAGE and RP-HPLC analysis.
Activity: One unit is defined as the amount of enzyme that will cleave 95% of 50I g fusion protein in 16 hours at 23a in 1x reaction buffer.
Recommended Enzyme Digestion Conditions
Fusion protein: 0.1mg
Recombinant enterokinase: 2U
10xbuffer: 10I l
Temperaturei a i : 2~8
Total volume: 100I l
Timei houri : 12~16
10x Reaction Buffer
500 mM Tris-HCl, pH 8.0
10 mM CaCl2
1% Tween-20 (v/v)
Stability: Stable at -20a , can be used up to 3 years.
Reference: LaVallie ER. et al.i 1993i J Biol Chem. Nov 5;268(31):23311-7.