Tryple Trypsin Elisa Kit

The kit uses anti TrypLE trypsin antibody as the coating antibody, the enzyme labeled anti TrypLE trypsin antibody as the detection antibody, the highly sensitive TMB as the substrate, and the TrypLE trypsin as the standard to quantitatively detect TrypLE trypsin in the sample. This kit is characterized by good specificity, high sensitivity and stability,
The lowest detectable level of TrypLE trypsin is 0.1ng/ml.
Principle
This kit uses double antibody sandwich method to detect TrypLE trypsin residuals. The TrypLE trypsin antibody is coated on the microplate, and the TrypLE trypsin in the sample or standard is combined with the antibody fixed on the microplate. After the unconjugated sample or standard is washed with washing solution, the HRP-labeled TrypLE trypsin antibody is added for incubation, and the enzyme labeled antibody is combined with TrypLE trypsin. Wash the unconjugated enzyme labeled antibody, add TMB substrate solution to the microplate, incubate it in dark to form a blue complex, stop the reaction with stop solution, and the color changes from blue to yellow. Determine the absorbance value of the complex, and fit the standard curve with the OD value of the standard. The TrypLE trypsin concentration of the sample can be calculated from the OD value of the sample through the formula fitted with the standard curve.
Kit components
ComponentsQTYPackage
Anti- TrypLE trypsin antibody coated microtiter plate12 strips96T
TrypLE trypsin STD5 vials1ml/vial
TrypLE trypsin reference1 vials1ml/vial
Sample dilution buffer1 bottle10ml/bottle
HRP-labeled antibodyi 100Xi 1 vial100I l/vial
HRP-labeled antibody dilution buffer1 bottle10ml/bottle
Substrate solution1 bottle10ml/bottle
Stop solution1 bottle10ml/bottle
Wash buffer concentratei 20Xi 2 bottle25ml/bottle
COA1 copy
Manual1 copy
Specificity
Control absi NSBi <0.300
3.2ng/ml std abs >0.700
Precisioni %CV average<9.0%
Detection limit (>control 3SD) <0.1ng/ml
Storage and stability
Antibody-coated plate, HRP labeled antibody and TrypLE trypsin standards are stored at -20a , other components are stored at 2-8a . It is stable for 6 months.
Catalog #
TE-19-007