12163 Qiagen Plasmid Maxi Kit (25)

The QIAGEN Plasmid Maxi Kit (catalog number 12163) is designed for the purification of up to 500 g of transfection-grade plasmid or cosmid DNA per preparation. This kit includes 25 QIAGEN-tip 500 columns, along with the necessary reagents and buffers. It employs gravity-flow anion-exchange chromatography to achieve high-purity DNA, comparable to two rounds of CsCl gradient centrifugation. The purified DNA is suitable for applications such as transfection, cloning, sequencing, and in vitro transcription. For optimal results, it's recommended to process 100500 ml of bacterial culture, depending on the plasmid copy number. citeturn0search0

For detailed protocols and additional information, please refer to the QIAGEN Plasmid Purification Handbook. citeturn0search3