Ni-nta Agarose 500 Ml

The 30250 Ni-NTA Agarose (500 mL) by Qiagen is a high-quality affinity resin designed for large-scale purification of His-tagged proteins under both native and denaturing conditions. Utilizing nickel-nitrilotriacetic acid (Ni-NTA) agarose, this resin provides high specificity and superior binding capacity, ensuring efficient capture and minimal non-specific interactions for highly pure protein isolation. The robust cross-linked agarose matrix ensures excellent mechanical stability, making it suitable for gravity-flow, batch purification, and FPLC-based chromatography. With a binding capacity of up to 50 mg of His-tagged protein per milliliter of resin, it offers reliable and reproducible purification even in high-throughput workflows. The resin is compatible with a range of imidazole concentrations for optimized elution and can be easily regenerated for multiple purification cycles. Ideal for large-scale protein expression, biochemical research, and structural studies, Qiagens Ni-NTA Agarose (500 mL) provides exceptional yield and purity for His-tagged protein purification.