Laboratory Use 11910 Ultrapure 100 Buffer Set

The Ultrapure 100 Buffer Set (catalog number 11910) is a comprehensive collection of buffers designed for use with QIAGEN's Ultrapure 500 Resin. This set facilitates the purification of up to 100 mg of ultrapure, endotoxin-free plasmid DNA, containing less than 0.1 endotoxin units (EU) per microgram of DNA. The buffers employ QIAGEN's anion-exchange technology, integrated with endotoxin removal, to achieve DNA purity comparable to or exceeding that obtained through two rounds of CsCl gradient centrifugation. citeturn0search0

Kit Components:

Buffer P1: 4 x 500 mL
Buffer P2: 4 x 500 mL
Buffer P3: 4 x 500 mL
Buffer QBT: 500 mL
Buffer QC: 6 x 500 mL
Buffer QN: 510 mL
Endotoxin-Free Water: 17 mL
Buffer TE: 240 mL
Buffer ER: 500 mL
RNase A: 4 x 50 mg

These components are sufficient for processing up to 100 mg of plasmid DNA.
Storage Conditions:
The Ultrapure 100 Buffer Set should be stored dry at room temperature (1525C). Under these conditions, the buffers remain stable for up to two years. After adding RNase A to Buffer P1, store it at 28C, where it remains stable for six months.
Applications:
This buffer set is ideal for applications requiring high-purity, endotoxin-free plasmid DNA, such as:
Transfection
Gene therapy research
Plasmid-mediated gene silencing
The integrated endotoxin removal step ensures that the purified DNA is suitable for sensitive downstream applications.
Important Considerations :
Endotoxin-Free Conditions: To maintain endotoxin-free conditions, use pyrogen-free plasticware or heat glassware to 180C overnight.
Buffer Preparation: Ensure all buffers are equilibrated to room temperature before use, except for Buffer P3, which should be prechilled to 4C.
RNase A Addition : Add RNase A to Buffer P1 to a final concentration of 100 g/mL before use.
For detailed protocols and additional information, refer to the Ultrapure 100 Handbook.

Brand : QIAGEN
Quality : Optimum
Grade : Laboratory
Condition : New
No. Of Kit : 100