Tris Hydrochloride (Tris Hcl)

The molecular weight of Tris was 121.14:0.1mol/l
The solution was 12.114g/l.
It can be absorb carbon dioxide from the air, so the bottle should be tightly closed.
It's certain pH value of 0.05mol/l
It can be prepared first, and then diluted to the required concentration.

2a 1M Tris-Hcli PH7.4a PH7.6a PH8.0i

Component concentration 1m Tris HCl

Configuration quantity 1L

Configuration method:

1a Weigh 121.1g Tris and put it in a 1 i L l beaker;

2a Add about 800 ml of deionized water and fully stir to dissolve;

3a Add concentrated HCl according to the following value to adjust the required pH value;

PH value concentrated HCl

7.4 about 70 ml

6, about 60ml

0, about 42ml

4a Volume the solution to 1 L;

5a After high temperature and high pressure sterilization, store at room temperature.

3a 1.5M Tris-Hcli PH8.8i

Component concentration 1.5m Tris HCl
Configuration quantity 1L

Configuration method:
Weigh 181.7g Tris and put it in 1L beaker;
Add about 800 ml of deionized water and fully stir to dissolve;
Adjust pH value to 8.8 with concentrated HCl;
Volume the solution to 1 L;

After high temperature and high pressure sterilization, store at room temperature.