Lab 12965 Qiagen Plasmid Plus Maxi Kit (100)

The 12965 QIAGEN Plasmid Plus Maxi Kit (100) is a high-performance plasmid DNA isolation kit from QIAGEN. This kit is optimized for Maxi-scale plasmid DNA preparation, offering high yields of highly purified plasmid DNA from bacterial cultures. It's suitable for high-throughput labs or large-scale projects that require substantial quantities of plasmid DNA.

Key Information:
Manufacturer : QIAGEN
Product Name QIAGEN Plasmid Plus Maxi Kit
Catalog Number : 12965
Capacity : The "100" in the product name indicates that this kit is designed for 100 plasmid preparations, typically from 250 mL bacterial cultures per prep.

Features:
High-Yield Plasmid DNA Purification: This "Plus" version of the kit is optimized for maximizing the yield and purity of plasmid DNA compared to standard Maxi kits. It's ideal for large-scale plasmid isolation, where high DNA quantities are necessary.
Large-Scale Capacity: The kit is specifically designed for processing larger bacterial cultures (250 mL), and its large pack size (100 preps) is perfect for labs with high throughput needs.
Purity and Integrity: The kit ensures that plasmid DNA is free of contaminants such as genomic DNA, RNA, and proteins. This makes the DNA highly suitable for sensitive applications like sequencing, transfection, and protein expression.
Applications:
Cloning: Perfect for large-scale cloning projects where substantial amounts of plasmid DNA are required.
Transfection: Suitable for transfection into a variety of eukaryotic cells (e.g., mammalian, insect cells) for gene expression or functional assays.
Gene Expression: Used for the preparation of plasmid DNA for recombinant protein production in bacteria or eukaryotic systems.
Sequencing: Ensures high-purity plasmid DNA suitable for sequencing applications, providing clean and reliable results.
Gene Therapy: Ideal for preparing large amounts of plasmid DNA used in gene therapy applications or other genetic manipulations.
Process Overview:
Bacterial Culture: Grow bacterial cultures (typically 250 mL) containing the plasmid of interest.
Cell Lysis: The cells are lysed to release plasmid DNA, which is then separated from genomic DNA and other cellular debris.
Plasmid DNA Binding: The plasmid DNA binds to a silica-based membrane or column under high-salt conditions.
Washing and Elution: Contaminants are removed during washing steps, and the purified plasmid DNA is eluted in an appropriate buffer for use in downstream applications.
Benefits:
Large Scale and High Yield: This kit provides an ideal solution for researchers who need large quantities of high-quality plasmid DNA for various applications.
Optimized for Purity: It ensures that the plasmid DNA is free from contaminants like genomic DNA, RNA, and proteins, which is essential for sensitive applications.
Cost-Effective for High-Throughput: The 100-prep capacity makes it an economical choice for labs that regularly perform plasmid DNA isolation on a large scale.
Versatility: Suitable for a wide range of molecular biology applications, including cloning, sequencing, protein expression, and transfection.
Ideal Use Case:
High-Throughput and Large-Scale Projects: The 12965 QIAGEN Plasmid Plus Maxi Kit (100) is perfect for labs or researchers needing to prepare large volumes of plasmid DNA from many bacterial cultures, making it ideal for large-scale cloning, protein expression, or functional genomics studies.
This kit is especially useful for labs working on gene therapy, high-throughput cloning, or other projects requiring high yields of purified plasmid DNA.