Lab 12963 Qiagen Plasmid Plus Maxi Kit (25)

The 12963 QIAGEN Plasmid Plus Maxi Kit (25) is a high-quality plasmid DNA isolation kit produced by QIAGEN, designed for Maxi-scale preparations. This kit is optimized to provide large yields of highly purified plasmid DNA, making it suitable for a wide range of molecular biology applications that require substantial quantities of plasmid DNA.

Key Information:
Manufacturer : QIAGEN
Product Name : QIAGEN Plasmid Plus Maxi Kit
Catalog Number : 12963
Capacity : The "25" in the product name indicates that this kit can process 25 plasmid preparations, typically from 250 mL bacterial cultures per prep.
Features:
High-Yield Plasmid DNA Purification: The "Plus" designation means that this kit is optimized for higher yields and superior purity of plasmid DNA compared to standard Maxi kits. This makes it particularly useful for labs that need large amounts of high-quality plasmid DNA for downstream applications.
Maxi-Scale Preparations: The kit is designed for processing large volumes (typically 250 mL) of bacterial culture, making it ideal for generating large quantities of plasmid DNA.
Purity: The kit ensures that the plasmid DNA is free from contaminants like genomic DNA, RNA, and proteins, ensuring the quality required for sensitive applications such as transfection and sequencing.
Applications:
Cloning: Ideal for large-scale cloning experiments that require high quantities of purified plasmid DNA.
Transfection: The high-quality plasmid DNA produced by this kit is suitable for transfection into a variety of eukaryotic cells, including mammalian and insect cells.
Gene Expression: This kit can be used to prepare plasmids for recombinant protein expression, gene therapy research, or functional genomics studies.
Sequencing: The purified plasmid DNA is suitable for sequencing applications, where high purity and integrity are critical for obtaining reliable results.
Large-Scale Studies: Used in studies that require large quantities of plasmid DNA, including those involving in vitro transcription, gene expression, and large-scale functional assays.
Process Overview:
Bacterial Culture: Grow 250 mL of bacteria that contain the plasmid of interest.
Cell Lysis: The cells are lysed, releasing plasmid DNA while precipitating out chromosomal DNA and other cellular debris.
Plasmid DNA Binding: The plasmid DNA binds to a silica-based membrane or column, while contaminants are washed away.
Elution: The purified plasmid DNA is then eluted in an appropriate buffer, ready for downstream applications.
Benefits:
High Yield and Purity: The kit is designed to provide high yields of high-quality plasmid DNA, making it ideal for applications that require significant amounts of DNA.
Optimized for Large-Scale Preps: This Maxi kit is ideal for large-scale plasmid production, making it suitable for research requiring substantial quantities of plasmid DNA.
Versatile: The kit can be used for a variety of molecular biology applications, including cloning, transfection, sequencing, and gene expression.
Ideal Use Case:
High-Throughput Labs: The 12963 QIAGEN Plasmid Plus Maxi Kit is ideal for labs that need to prepare plasmid DNA from a large number of bacterial cultures (25 preps) and need large amounts of purified plasmid for multiple downstream applications.
This kit is particularly useful for researchers working on projects that require high-quality, large quantities of plasmid DNA, such as gene expression studies, protein production, or high-throughput functional genomics.