12943 Qiagen Plasmid Plus Midi Kit (25) Lab Use

The 12943 QIAGEN Plasmid Plus Midi Kit (25) is a plasmid DNA isolation kit from QIAGEN, designed for efficiently extracting high-quality plasmid DNA from bacterial cultures. This kit is optimized for Midi-scale preparations, providing a higher yield and purity compared to standard kits.

Key Information:
Manufacturer : QIAGEN
Product Name : QIAGEN Plasmid Plus Midi Kit
Catalog Number : 12943
Capacity : The "25" indicates that the kit can process 25 plasmid preparations, typically from 50 mL bacterial cultures per prep.

Features:
High Yield and Purity: The "Plus" designation signifies that this kit is optimized for higher yields and purity of plasmid DNA compared to standard Midi kits, making it suitable for demanding applications.
Efficient Plasmid Isolation: The kit is designed to recover plasmid DNA from bacterial cultures in a more efficient manner, which is particularly useful for larger-scale preparations or for difficult-to-isolate plasmids.
Easy-to-Follow Protocol: The kit includes reagents and protocols that are optimized for ease of use, providing a streamlined process for plasmid isolation.
Applications:
Cloning: The high-quality plasmid DNA obtained can be used for cloning experiments, including the insertion of new genetic material.
Transfection: Suitable for introducing plasmid DNA into mammalian, insect, or other eukaryotic cells for gene expression studies.
Sequencing: The kit yields high-purity plasmid DNA that can be used in sequencing applications, where purity is critical for obtaining accurate results.
Gene Expression Studies: Ideal for preparing plasmids for use in protein expression, gene silencing, or other genetic manipulation applications.
Process Overview:
Bacterial Growth: A bacterial culture (typically 50 mL) carrying the plasmid is grown in a suitable liquid medium.
Cell Lysis: The cells are lysed to release plasmid DNA, followed by neutralization to precipitate chromosomal DNA and cellular debris.
Plasmid DNA Binding: The plasmid DNA binds to a silica-based column or membrane, while contaminants are removed during washing steps.
Elution: The purified plasmid DNA is eluted in a clean buffer, ready for use in downstream applications.
Benefits:
Increased Yield: The kit ensures a higher yield of plasmid DNA, ideal for applications that require larger amounts of DNA, such as transfection and large-scale cloning.
High Purity: The optimized protocol ensures that the plasmid DNA is of high quality, which is essential for sensitive applications like sequencing or transfection.
Versatile: This kit is suitable for a wide range of applications, from cloning to gene expression to sequencing.
This kit is particularly useful for labs needing a reliable, high-yield plasmid preparation method for various molecular biology workflows.