12863 Compactprep Plasmid Maxi Kit (25)

The 12863 CompactPrep Plasmid Maxi Kit (25) is another plasmid DNA extraction kit by Qiagen, designed for isolating larger quantities of plasmid DNA from bacterial cultures. Heres a breakdown of the product:

Key Information:

• Manufacturer: Qiagen
• Product Name: CompactPrep Plasmid Maxi Kit
• Catalog Number: 12863
• Capacity: The "25" indicates that the kit is designed to process up to 25 plasmid DNA preparations (typically from larger bacterial cultures, around 250 mL per prep).

Features:

• High-Yield Plasmid Purification: The CompactPrep Plasmid Maxi Kit is optimized for isolating large amounts of high-quality plasmid DNA, making it ideal for applications requiring significant plasmid quantities.
• Quick and Efficient: The protocol is streamlined for ease of use and rapid processing, providing high yields of plasmid DNA with minimal time and effort.
• Suitable for Large-Scale Preps: This kit is specifically designed for "Maxi" preparations, where you need larger amounts of plasmid DNA, which is essential for transfections, large-scale cloning, or in vitro transcription.

Applications:

• Transfection: Suitable for introducing large quantities of plasmid DNA into eukaryotic cells, including mammalian and insect cells.
• Protein Expression: Often used for preparing plasmid DNA for the expression of recombinant proteins.
• Sequencing: Ensures high-quality plasmid DNA for sequencing applications, where high purity is crucial.
• Gene Therapy: Used in applications that require large quantities of plasmid DNA for gene therapy research.

Process Overview:

1. Bacterial Culture: Grow a large volume (usually 250 mL) of bacteria carrying the plasmid.
2. Cell Lysis: Cells are lysed to release plasmid DNA.
3. DNA Binding: The plasmid DNA binds to a silica membrane.
4. Washing and Elution: The plasmid DNA is washed to remove contaminants, then eluted into a clean buffer.