Cis-repressed Pqe Kan Vector Set - 500g, N-terminal 6xhis Tag | Tight Expression Control, Kanamycin Resistance, Three Reading Frames Provided

Condition : New

Grade : Laboratory

Brand : QIAGEN

Tag : 6xHis tag

Antibiotic resistance : cis-Repressed pQE Vector Set: Ampicillin; cis-Repressed pQE KAN Vector Set: Kanamycin

Expression species : E. coli

N- or C-terminal tag : N-terminal tag

Expression : In vivo

Special features : Additional expression of the laclq gene

In-frame cloning necessary : Yes

Tag removal sequence : No

All three reading frames provided : Yes

Supplier Location : Kurukshetra, Haryana, India

The 32943 cis-Repressed pQE Kan Vector Set by Qiagen is designed for tight control of protein expression in E. coli, reducing background expression while enabling high-yield production of His-tagged proteins upon induction. This vector set includes three expression vectorspQE-80L, pQE-81L, and pQE-82Leach containing a cis-repressed T5 promoter regulated by the lac operator (lacO). This design minimizes leaky expression, making it ideal for toxic or unstable proteins that require strict regulation before induction.

The vectors allow for N-terminal His-tag fusion, enabling efficient purification via Ni-NTA affinity chromatography and easy detection using anti-His antibodies. They also feature a kanamycin resistance gene (KanR), offering an alternative to ampicillin selection and reducing the risk of satellite colony formation.

The cis-Repressed pQE Kan Vector Set is particularly useful for functional protein studies, enzymatic assays, and structural biology, ensuring precise, controlled, and high-yield recombinant protein expression in bacterial systems.