Lab 12961 Qiagen Plasmid Plus Maxi Sample Kit (5)

The 12961 QIAGEN Plasmid Plus Maxi Sample Kit (5) is a QIAGEN product designed for the isolation of high-quality plasmid DNA from bacterial cultures at a Maxi scale. This kit is optimized for larger plasmid preparations, providing high yields of purified plasmid DNA suitable for a variety of downstream applications.

Key Information:
Manufacturer : QIAGEN
Product Name : QIAGEN Plasmid Plus Maxi Sample Kit
Catalog Number : 12961
Capacity : The "5" indicates that this kit can process 5 plasmid preparations, typically from 250 mL bacterial cultures per prep.

Features:
High-Yield Plasmid Isolation: The "Plus" version of this kit is optimized for maximizing both the yield and purity of plasmid DNA compared to standard Maxi kits. It is especially useful for larger-scale preparations where high DNA yield is critical.
Maxi-Scale Purification: This kit is designed for large-scale plasmid DNA preparation (250 mL bacterial culture) and is ideal for applications requiring large amounts of plasmid DNA.
Purity and Integrity: The kit provides high-quality plasmid DNA that is free from contaminants, such as genomic DNA, RNA, and proteins, making it suitable for sensitive applications.
Applications:
Cloning: Ideal for preparing large quantities of plasmid DNA for cloning experiments, where you need a substantial amount of DNA for insertions or other manipulations.
Transfection: This kit is suitable for preparing plasmid DNA for transfection into eukaryotic cells, especially mammalian cells, which require higher DNA concentrations for successful transfection.
Protein Expression: Used for preparing plasmids for protein expression in bacteria or eukaryotic cells.
Sequencing: Purified plasmid DNA is ready for sequencing applications, ensuring clean, accurate results.
Gene Therapy: Ideal for preparing large amounts of plasmid DNA needed in gene therapy studies or other genetic research.
Process Overview:
Bacterial Growth: Grow 250 mL of bacterial culture containing the plasmid.
Cell Lysis and DNA Extraction: Cells are lysed, and plasmid DNA is separated from genomic DNA and other contaminants through a series of steps.
Binding: The plasmid DNA binds to a silica membrane under high salt conditions.
Washing and Elution: Contaminants are washed away, and the plasmid DNA is eluted in an appropriate buffer for downstream use.
Benefits:
High Yields: This Maxi kit is designed to yield large amounts of high-quality plasmid DNA, making it ideal for applications that require substantial DNA quantities.
Optimized for Purity: The kit ensures that the plasmid DNA is highly pure, free of genomic DNA, RNA, and protein contaminants.
Suitable for Sensitive Applications: The high purity and yield make this kit perfect for demanding applications such as transfection, sequencing, and gene therapy.
Versatile: This kit is applicable for a wide range of molecular biology workflows and applications.
Ideal Use Case:
Large-scale Plasmid Production: The 12961 Plasmid Plus Maxi Sample Kit is perfect for research projects that require large amounts of plasmid DNA, such as those involving high-throughput transfections, large-scale cloning, or preparing DNA for in vitro transcription.