Bio-technology Institute

Bio-technology InstituteMicro-Biology One MonthsB.Sc./M.Sc/ B.Tech./M.Tech. Biotechnology, Microbiology, Biochemistry,Agricultural Biotechnology, Food Biotechnology and other field of life Sciences. Training objectsBasic Laboratory Rules,Fundamentals of Laboratory Training Demonstration. Preparation of Temporary and Permanent Cotton plug, Basic requirement of a Microbiology Laboratory.Preparation of Important laboratory chemical & Buffers.Methods of Sterilization.Moist heatDry heat FiltrationSurface SterilizationRadiation Preparation of Culture Media Solid media (agar)Agar slantsAgar deep tubesIntroduction of Quality Control of media.pH measurement of solution & culture Media.Sampling Techniques Sampling of Air Sampling of Water Sampling of Soil Culture methods/Plating Techniques of MicroorganismsStreak plate methodPour plate methodSpread plate methodSerial Dilution agar plate method.Methods of culture preservation & MaintenanceLyophilizationStorage in Liquid NitrogenStorage in SoilStorage in gelatin discs By sub culturingStorage in mineral oilMeasurement of Microorganism. Culture and Subculture. Micro-Biology Two MonthsMolecular Biology One MonthsB.Sc./M.Sc/
B.Tech./M.Tech. Biotechnology, Microbiology, Biochemistry,Agricultural
Biotechnology, Food Biotechnology and other field of life Sciences. Training objectsBasic Laboratory Rules,Fundamentals of Laboratory Training Demonstration. Preparation of Temporary and Permanent Cotton plug, Basic requirement of a Microbiology Laboratory.Preparation of Important laboratory chemical & Buffers.Methods of Sterilization.Moist heatDry heat FiltrationSurface SterilizationRadiation Preparation of Culture Media Solid media (agar)Agar slantsAgar deep tubesIntroduction of Quality Control of media.pH measurement of solution & culture Media.Sampling Techniques Sampling of Air Sampling of Water Sampling of Soil Culture methods/Plating Techniques of MicroorganismsStreak plate methodPour plate methodSpread plate methodSerial Dilution agar plate method.Methods of culture preservation & MaintenanceLyophilizationStorage in Liquid NitrogenStorage in SoilStorage in gelatin discs By sub culturingStorage in mineral oilMeasurement of Microorganism. Culture and Subculture. Different type of Media preparation.Bacterial Cell Counting & Staining Techniques.Method for detection of specific MicroorganismThesis Preparation  Micro-Biology Three Months Biotechnology Six MonthB.Sc. /M.Sc / B.Tech. /M.Tech. Biotechnology, Microbiology,
Biochemistry, Agricultural Biotechnology, Food Biotechnology and
other field of life Sciences.Dissertation work depends on their Syllabus Micro-Biology Six MonthsBiotechnology Six Month
B.Sc. /M.Sc / B.Tech. /M.Tech, Biotechnology, Microbiology,
Biochemistry, Agricultural Biotechnology, Food Biotechnology and
other field of life Sciences.Dissertation work depends on their Syllabus Biochemistry One Month B.Sc./M.Sc/ B.Tech./M.Tech. Biotechnology, Microbiology, Biochemistry,Agricultural Biotechnology, Food Biotechnology and other field of life Sciences.Training objectsBasic Laboratory Rules,Fundamentals of Laboratory Training Demonstration.Preparation of Temporary and Permanent Cotton plug,Basic requirement of a Bio-chemistry Laboratory.Preparation of Important laboratory chemical & Buffers.Methods of Sterilization.Method of using Balance.pH measurement of solution & culture Media.CarbohydrateDetermination of total Carbohydrate from given sample.Determination of starch from given SampleDetermination of sugar with DNS reagentsExtraction of sugar from given SampleLipidQualitative test on lipidDetermination of oil content from grain/ oil Seed.Separation of Phospholipids by TLCAmino AcidQualitative test on amino acid & protein.Isolation of Casein from milk.Determination of Amino Acid from given Sample.Nucleic AcidQualitative test on Nucleic AcidIsolation of Nucleic Acid from given Sample.Separating technique in Biochemistry by Centrifuge. Biochemistry Two MonthsB.Sc./M.Sc/ B.Tech./M.Tech. Biotechnology, Microbiology, Biochemistry,Agricultural Biotechnology, Food Biotechnology and other field of life Sciences. Training objectsOne Month Module In Biotechnology Molecular BiologyDNA isolation from Plant SourceDNA isolation from animal sourceDNA isolation from Bacterial source.Agarose gel electrophoresis.PCR.MicrobiologyIsolation of MicrobesBy pour plate techniqueBy spread plate techniquePurification of Microorganisms by Different streaking techniques.Preservation of purified culturesGrams Staining for BacteriaLacto phenol Cotton blue staining for fungiStudy of bacterial growth curveAntibiotic assay.EnzymologyIsolation of microbes from suspected sources for production of particular enzymeScreening of cultures for enzyme productionProduction /Fermentation for enzyme productionPreparation of crude enzymeProtein estimation by Lowry's methodSalt precipitationDialysisIon exchange chromatographyEnzyme assaySDS PAGEImmobilization of enzymePlant Tissue CultureIntroduction of plant tissue cultureInitiation of explantsEmbryo CultureBiochemistry Three Months Biotechnology Six MonthB.Sc. /M.Sc / B.Tech. /M.Tech, Biotechnology, Microbiology,
Biochemistry, Agricultural Biotechnology, Food Biotechnology and
other field of life Sciences.Dissertation work depends on their Syllabus Biochemistry Six Months Biotechnology Six Month
B.Sc. /M.Sc / B.Tech. /M.Tech, Biotechnology, Microbiology,
Biochemistry, Agricultural Biotechnology, Food Biotechnology and
other field of life Sciences.
Dissertation work depends on their Syllabus Molecular Biology One MonthB.Sc./M.Sc/ B.Tech./M.Tech. Biotechnology, Microbiology, Biochemistry,Agricultural Biotechnology, Food Biotechnology and other field of life Sciences. Training objectsBasic Laboratory Rules,Fundamentals of Laboratory Training Demonstration. Preparation of Temporary and Permanent Cotton plug, Basic requirement of a molecular Biology Laboratory.Preparation of Important laboratory chemical & Buffers.Methods of Sterilization.Method of using Balance.pH measurement of solution & culture Media.Isolation of DNA from given sample.Isolation of Plasmid DNA by using Alkaline Lyses Methods.Preparation of genomic DNA.Report Preparation. Molecular Biology Two MonthMolecular Biology One Month B.Sc./M.Sc/
B.Tech./M.Tech. Biotechnology, Microbiology, Biochemistry,Agricultural
Biotechnology, Food Biotechnology and other field of life Sciences. Training objects Basic Laboratory Rules,Fundamentals of Laboratory Training Demonstration.Preparation of Temporary and Permanent Cotton plug,Basic requirement of a molecular Biology Laboratory.Preparation of Important laboratory chemical & Buffers.Methods of Sterilization.Method of using Balance.pH measurement of solution & culture Media.Preparation of Agarose gelPreparation of Acryl amide gel  Isolation of DNA from given sample.Isolation of Plasmid DNA by using Alkaline Lyses Methods.Preparation of genomic DNA and Demonstrations Isolation of Protein from given Sample.Staining of gel. Report Preparation.Molecular Biology Three Month Biotechnology Six Month
B.Sc. /M.Sc / B.Tech. /M.Tech, Biotechnology, Microbiology,
Biochemistry, Agricultural Biotechnology, Food Biotechnology and
other field of life Sciences.
Dissertation work depends on their SyllabusMolecular Biology Six Month 
Biotechnology Six Month

B.Sc. /M.Sc / B.Tech. /M.Tech, Biotechnology, Microbiology,
Biochemistry, Agricultural Biotechnology, Food Biotechnology and
other field of life Sciences.

Dissertation work depends on their SyllabusBiotechnology One MonthBasic Laboratory techniques , Bioinstrumentation, and aspects related to maintaining aseptic environment. Molecular BiologyDNA Isolation from plants.DNA isolation from bacteria.Purification of DNA By electrophoresis technique.MicrobiologyIsolation of Microbes from various sources.Purification of Microbes.Preservation of pure cultures.Identification of microbes.Plant Tissue CultureIntroduction of plant tissue cultureInitiation of explantsEstablishment of cultures.Embryo CultureEnzymologyScreening of enzymesIsolation of enzymes/ProteinProtein estimation Biotechnology Two MonthB.Sc/M.Sc/B.tech/M.tech. Molecular Biology,Biotechnology,Micro
Biology,Biochemistry,Agricultural Biotechnology and Food Biotechnology
and other field of Life SciencesMolecular BiologyDNA Isolation from plants.DNA Isolation from animalsDNA isolation from bacteria.Agarose gel electrophoresisPCR MicrobiologyIsolation of MicrobesPurification of Microbes.Preservation of pure cultures.Bacterial stainingFungal stainingStudy of bacterial growth curveAntibiotic assay EnzymologyIsolation of microbesScreening of cultures for enzyme productionEnzyme productionPreparation of crude enzymeProtein estimation by Lowry's methodSalt precipitationDialysisIon exchange chromatographyEnzyme assaySDS PAGEImmobilization of enzyme

Plant Tissue CultureIntroduction of plant tissue cultureInitiation of explantsEstablishment of cultures.Embryo CultureEnzymology One MonthB.Sc./M.Sc/
B.Tech./M.Tech. Biotechnology, Microbiology, Biochemistry,Agricultural
Biotechnology, Food Biotechnology and other field of life Sciences. Training objectsIntroduction to Enzymology.Sterilization Techniques.Isolation of Microorganisms from natural sources.Pure culture Preparation.Screening of purified cultures for Enzyme activity.Identification of unknown microorganisms.Fermentation.Downstream Processing.Extraction of Crude Enzyme.Purification of enzyme.Precipitation of Enzyme.Dialysis.Ion Exchange Chromatography.Thin Layer Chromatography (depending on enzyme). Enzymology Two MonthB.Sc./M.Sc/ B.Tech./M.Tech. Biotechnology, Microbiology, Biochemistry,Agricultural Biotechnology, Food Biotechnology and other field of life Sciences. Training objectsIntroduction to Enzymology.Sterilization Techniques.Isolation of Microorganisms from natural sources.Pure culture Preparation.Screening of purified cultures for Enzyme activity.Identification of unknown microorganisms.Fermentation.Downstream Processing.Extraction of Crude Enzyme.Purification of enzyme.Precipitation of Enzyme.Dialysis.Ion Exchange Chromatography.Thin Layer Chromatography (depending on enzyme).Characterization of Purified EnzymeTotal protein estimationBradford's Method.Lowry's Method.280 Methods.Enzyme AssayEnzyme KineticsEffect of pH on Enzyme ActivityEffect of Temperature on Enzyme ActivityEffect of Substrate Concentration on Enzyme ActivityEffect of Activator on Enzyme ActivityNative PAGE (IDEA) Enzymology Three MonthEnzymology Two Month B.Sc./M.Sc/
B.Tech./M.Tech. Biotechnology, Microbiology, Biochemistry,Agricultural
Biotechnology, Food Biotechnology and other field of life Sciences. Training objectsIntroduction to Enzymology.Sterilization Techniques.Isolation of Microorganisms from natural sources.Pure culture Preparation.Screening of purified cultures for Enzyme activity.Identification of unknown microorganisms.Fermentation.Downstream Processing.Extraction of Crude Enzyme.Purification of enzyme.Precipitation of Enzyme.Dialysis.Ion Exchange Chromatography.Thin Layer Chromatography (depending on enzyme).Characterization of Purified EnzymeTotal protein estimationBradford's Method.Lowry's Method.280 Methods.Enzyme AssayEnzyme KineticsEffect of pH on Enzyme ActivityEffect of Temperature on Enzyme ActivityEffect of Substrate Concentration on Enzyme ActivityEffect of Activator on Enzyme ActivityNative PAGE (IDEA) SDS PAGEZymography (IDEA)  Immobilization of EnzymesEnzymology Six MonthBiotechnology Six Month

B.Sc. /M.Sc / B.Tech. /M.Tech, Biotechnology, Microbiology,
Biochemistry, Agricultural Biotechnology, Food Biotechnology and
other field of life Sciences.

Dissertation work depends on their Syllabus  Plant Tissue Culture One MonthsB.Sc./M.Sc/ B.Tech./M.Tech. Biotechnology, Microbiology, Biochemistry,Agricultural Biotechnology, Food Biotechnology and other field of life Sciences. Training objectsIntroduction of institute.Laboratory visit.Introduction of plant tissue culture lab.Importance of Green house.Introduction to Plant Biotechnology.History of plant tissue culture.Application of plant tissue culture.Sterilization & Types of sterilization.Washing of glassware.Process of contaminated media discardation.Demonstration of Embryo culture.Media preparation for initiation explants.Media preparation for multiplication of culture.Media preparation for shooting of culture.Media preparation for rooting of culture.Checking of culture media.Selection of mother plant.Isolation of meristem.Sterilization of explantsInitiation of plants meristematic tissue.  

 
Plant Tissue Culture Two MonthsB.Sc./M.Sc/
B.Tech./M.Tech. Biotechnology, Microbiology, Biochemistry,Agricultural
Biotechnology, Food Biotechnology and other field of life Sciences. Training objectsIntroduction of institute.Laboratory visit.Introduction of plant tissue culture lab.Importance of Green house.Introduction to Plant Biotechnology.History of plant tissue culture.Application of plant tissue culture.Sterilization & Types of sterilization.Washing of glassware.Process of contaminated media discardation.Demonstration of Embryo culture.Media preparation for initiation explants.Media preparation for multiplication of culture.Media preparation for shooting of culture.Media preparation for rooting of culture.Checking of culture media.Selection of mother plant.Isolation of meristem.Sterilization of explantsInitiation of plants meristematic tissue. Multiplication of culture.Shooting of culture.Rooting of culture.Checking of cultures.Submission of report Plant Tissue Culture Three MonthsB.Sc./M.Sc/
B.Tech./M.Tech. Biotechnology, Microbiology, Biochemistry,Agricultural
Biotechnology, Food Biotechnology and other field of life Sciences. Training objectsIntroduction of institute.Laboratory visit.Introduction of plant tissue culture lab.Importance of Green house.Introduction to Plant Biotechnology.History of plant tissue culture.Application of plant tissue culture.Sterilization & Types of sterilization.Washing of glassware.Process of contaminated media discardation.Demonstration of Embryo culture.Media preparation for initiation explants.Media preparation for multiplication of culture.Media preparation for shooting of culture.Media preparation for rooting of culture.Checking of culture media.Selection of mother plant.Isolation of meristem.Sterilization of explantsInitiation of plants meristematic tissue. Multiplication of culture.Shooting of culture.Rooting of culture.Checking of cultures.Primary hardening of cultured plants.Secondary hardening of plants.Submission of assignment topic.Presentation on seminar topic.Submission of report.Presentation Plant Tissue Culture Six Months Biotechnology Six Month

B.Sc. /M.Sc / B.Tech. /M.Tech, Biotechnology, Microbiology,
Biochemistry, Agricultural Biotechnology, Food Biotechnology and
other field of life Sciences.

Dissertation work depends on their Syllabus  Bioinformatics One Month Bioinformatics One & Two MonthB.Sc./M.Sc/ B.Tech./M.Tech. Biotechnology, Microbiology, Biochemistry,Agricultural Biotechnology, Food Biotechnology and other field of life Sciences. Training objectsIntroduction of Bioinformatics TechnologyApplication of computer in Bioinformatics ResearchGenomicsProteomicsData base StudiesDrug Designing Bioinformatics Two Month Bioinformatics One & Two MonthB.Sc./M.Sc/
B.Tech./M.Tech. Biotechnology, Microbiology, Biochemistry,Agricultural
Biotechnology, Food Biotechnology and other field of life Sciences. Training objectsIntroduction of Bioinformatics TechnologyApplication of computer in Bioinformatics ResearchGenomicsProteomicsData base StudiesDrug Designing Bioinformatics Three Month Biotechnology Six Month

B.Sc. /M.Sc / B.Tech. /M.Tech, Biotechnology, Microbiology,
Biochemistry, Agricultural Biotechnology, Food Biotechnology and
other field of life Sciences.

Dissertation work depends on their Syllabus Bioinformatics Six Month 
Biotechnology Six Month


B.Sc. /M.Sc / B.Tech. /M.Tech, Biotechnology, Microbiology,
Biochemistry, Agricultural Biotechnology, Food Biotechnology and
other field of life Sciences.



Dissertation work depends on their Syllabus   Bio Medical One MonthIntroduction Institute Training programme Laboratory rules and precautions Lists of instruments Uses of it Circuit description Working Handling of devices Visit Diagnostic centres Hospitals Report On project Biodiesel One MonthsB.Sc./M.Sc/ B.Tech./M.Tech. Biotechnology, Microbiology, Biochemistry,Agricultural Biotechnology, Food Biotechnology and other field of life Sciences. Training objectsBasic Laboratory Rules, Fundamentals of Laboratory Training Demonstration. Preparation of Temporary and Permanent Cotton plug, Basic requirement of a chemistry Laboratory.Preparation of Important laboratory chemical & Buffers.Methods of Sterilization. Method of using Balance.pH measurement of solution & culture Media.Introduction of Bio-diesel plants(Jatropha curcus)Biological / Bio-Energy feature of JatrophaBio-Prospects of Jatropha curcus in different waysBio-Chemistry of JatrophaPlants Developments Biodiesel Two MonthsB.Sc./M.Sc/
B.Tech./M.Tech. Biotechnology, Microbiology, Biochemistry,Agricultural
Biotechnology, Food Biotechnology and other field of life Sciences. Training objectsBasic Laboratory Rules,Fundamentals of Laboratory Training Demonstration. Preparation of Temporary and Permanent Cotton plug, Basic requirement of a chemistry Laboratory.Preparation of Important laboratory chemical & Buffers.Methods of Sterilization. Method of using Balance.pH measurement of solution & culture Media.Introduction of Bio-diesel plants(Jatropha curcus)Biological / Bio-Energy feature of JatrophaBio-Prospects of Jatropha curcus in different waysBio-Chemistry of JatrophaPlants Developments through Seed & Shoots.Plantation TechniqueIncome through plants.Bio-diesel production. Biodiesel Three MonthBiotechnology Six Month



B.Sc. /M.Sc / B.Tech. /M.Tech, Biotechnology, Microbiology,
Biochemistry, Agricultural Biotechnology, Food Biotechnology and
other field of life Sciences.




Dissertation work depends on their Syllabus  Biodiesel Six Month
Biotechnology Six Month




B.Sc. /M.Sc / B.Tech. /M.Tech, Biotechnology, Microbiology,
Biochemistry, Agricultural Biotechnology, Food Biotechnology and
other field of life Sciences.





Dissertation work depends on their Syllabus  Production Technology Biofertilizer Biotechnology Six Month





B.Sc. /M.Sc / B.Tech. /M.Tech, Biotechnology, Microbiology,
Biochemistry, Agricultural Biotechnology, Food Biotechnology and
other field of life Sciences.






Dissertation work depends on their Syllabus  Production Technology Biopestisides
Biotechnology Six Month






B.Sc. /M.Sc / B.Tech. /M.Tech, Biotechnology, Microbiology,
Biochemistry, Agricultural Biotechnology, Food Biotechnology and
other field of life Sciences.







Dissertation work depends on their Syllabus  Production Technology Mashroom
Biotechnology Six Month






B.Sc. /M.Sc / B.Tech. /M.Tech, Biotechnology, Microbiology,
Biochemistry, Agricultural Biotechnology, Food Biotechnology and
other field of life Sciences.












Dissertation work depends on their Syllabus