27115 Qiaprep 2.0 Spin Columns (100)

The 27115 QIAprep 2.0 Spin Columns (100) from Qiagen are designed for high-efficiency plasmid DNA purification in molecular biology applications. These columns are compatible with QIAprep Miniprep Kits and can be used to replace or supplement existing kits, offering flexibility and convenience for researchers handling multiple samples.

The spin columns utilize advanced silica membrane technology to selectively bind plasmid DNA while removing impurities such as proteins, salts, and cellular debris. The workflow involves alkaline lysis of bacterial cells, DNA binding to the membrane, thorough washing to eliminate contaminants, and elution of high-purity DNA in a low-salt buffer or water.

With a streamlined protocol taking around 10 minutes per prep, these columns yield up to 20 g of high-quality plasmid DNA, suitable for downstream applications like cloning, PCR, sequencing, and transfection. The QIAprep 2.0 Spin Columns deliver reliable and reproducible results, making them an essential tool for routine DNA purification in research labs.