12945 Qiagen Plasmid Plus Midi Kit (100) Lab Use

The 12945 QIAGEN Plasmid Plus Midi Kit (100) is a large-scale plasmid DNA isolation kit designed by QIAGEN for the purification of high-quality plasmid DNA from bacterial cultures. It is designed for Midi-scale preparations but offers a larger quantity of reagents to process up to 100 plasmid preparations, which is ideal for high-throughput labs or projects requiring significant amounts of plasmid DNA.

Key Information:
Manufacturer : QIAGEN
Product Name : QIAGEN Plasmid Plus Midi Kit
Catalog Number : 12945
Capacity : The "100" refers to the kits capacity to process 100 plasmid preparations, typically from 50 mL bacterial cultures per prep.

Features:
Optimized for High Yield and Purity: The "Plus" version of this kit is tailored to provide higher yields and improved purity of plasmid DNA compared to standard Midi kits. It is ideal for obtaining plasmid DNA from challenging bacterial strains or for projects requiring larger quantities of plasmid.
High Throughput: With 100 preparations, the kit is well-suited for labs that need to process a larger number of samples in a more cost-effective manner.
Efficient and Scalable: The kit's protocol is designed to allow scaling up, making it suitable for both small and large plasmid preparations. It provides a streamlined approach for isolating large quantities of plasmid DNA without sacrificing quality.
Applications:
Cloning: Suitable for cloning experiments where large amounts of plasmid DNA are needed.
Transfection: Ideal for preparing plasmid DNA for transfection into eukaryotic cells, such as mammalian or insect cells.
Gene Expression: Used for the production of plasmids to express recombinant proteins in vitro or in living organisms.
Sequencing: The high-quality plasmid DNA obtained from this kit is suitable for sequencing applications, ensuring that the sequencing results are accurate and reliable.
Process Overview:
Bacterial Growth: Grow a bacterial culture (typically 50 mL) containing the plasmid of interest.
Cell Lysis and DNA Extraction: The cells are lysed to release plasmid DNA, which is then separated from genomic DNA and cellular debris.
Binding: The plasmid DNA binds to a silica column or membrane under high salt conditions.
Washing and Elution: Contaminants are washed away, and the purified plasmid DNA is eluted in a clean buffer, ready for use in downstream applications.
Benefits:
High Yield: This kit is ideal for labs needing large quantities of plasmid DNA for various applications, ensuring higher yields without compromising purity.
High Purity: The kit ensures that plasmid DNA is free from contaminants like genomic DNA, RNA, and proteins, which is critical for sensitive downstream applications.
Versatility: Suitable for a wide range of molecular biology applications, from cloning to transfection to sequencing.
Cost-Effective: Designed for labs that need to process multiple samples, offering a more economical option when compared to purchasing individual kits.
This kit is an excellent choice for labs that regularly prepare plasmid DNA from large volumes of bacterial cultures, making it a good option for high-throughput applications or for researchers who need large quantities of purified plasmid DNA.