12391 Endofree Plasmid Giga Kit (5) Laboratory Chemical

The QIAGEN EndoFree Plasmid Giga Kit (5) is a premium kit designed for the isolation of high-purity, endotoxin-free plasmid DNA from very large bacterial cultures. It is particularly suited for applications that require large amounts of plasmid DNA and where endotoxin contamination is a concern, such as in gene therapy, vaccine development, and transfection.

Key Features:
Endotoxin-Free DNA: The kit ensures very low endotoxin levels in the purified plasmid DNA, making it ideal for sensitive applications such as gene therapy, mammalian cell transfection, and DNA vaccine production, where endotoxins can be harmful.
High Yield: The EndoFree Plasmid Giga Kit is designed for very large-scale plasmid isolation, with the ability to isolate up to 1.55 mg of plasmid DNA from up to 20 liters of bacterial culture. This makes it suitable for industrial-scale or high-throughput applications that require significant quantities of plasmid DNA.
High Purity : The kit is optimized to ensure that plasmid DNA is highly pure, free from contaminants such as RNA, proteins, and endotoxins, ensuring suitability for a wide range of downstream applications like transfection, sequencing, and protein expression.
Efficient Endotoxin Removal : The purification process effectively removes endotoxins during the plasmid isolation procedure, which is particularly important for sensitive biological applications. Streamlined Workflow : The kit uses a modified alkaline lysis method combined with QIAGEN's proprietary silica-based chromatography, making the protocol faster and more efficient than traditional methods, with fewer steps and reduced hands-on time.
Components:
Buffers : The kit contains buffers for bacterial cell resuspension, lysis, neutralization, endotoxin removal, washing, and elution. These buffers are specifically formulated for efficient endotoxin removal and high plasmid yield. EndoFree Chromatography Columns : These columns facilitate the removal of endotoxins while purifying plasmid DNA, ensuring that only high-quality, endotoxin-free plasmid DNA is recovered.
Elution Solution : A buffer or water used to elute the purified plasmid DNA from the column.
Benefits:
Endotoxin-Free : Ideal for applications that require endotoxin-free plasmid DNA, such as gene therapy, cell transfection, and vaccine production, where endotoxins can interfere with results.
High Yield: Suitable for large-scale plasmid isolation, providing significant quantities of plasmid DNA from large bacterial cultures (up to 20 liters).
High Purity : Ensures that the plasmid DNA is of the highest quality, free from contaminants such as RNA, proteins, and endotoxins, making it ideal for downstream applications such as cloning, sequencing, and transfection.
Simplified Process : The QIAGEN method reduces the number of steps required for plasmid purification, making it faster and more efficient than traditional alkaline lysis methods.
Versatility : The kit works with a variety of bacterial strains and plasmid sizes, making it a versatile solution for different experimental needs.

Application :
Gene Therapy : The endotoxin-free plasmid DNA is ideal for gene therapy applications, where endotoxins can interfere with the success of treatment.
Transfection : High-quality, endotoxin-free plasmid DNA is crucial for successful transfection into mammalian cells.
Protein Expression : Suitable for large-scale recombinant protein production in bacterial or eukaryotic expression systems.