12362 Endofree Plasmid Maxi Kit (10) Laboratory Chemicals

The QIAGEN EndoFree Plasmid Maxi Kit (10) is a high-performance plasmid DNA isolation kit designed to yield high-quality plasmid DNA that is free of endotoxins, making it ideal for applications that require minimal contamination. This kit is specifically suited for large-scale preparations from bacterial cultures, particularly for applications like transfection, protein expression, or gene therapy where endotoxins can interfere with results.

Key Features:
Endotoxin-Free DNA: This kit is designed to isolate plasmid DNA with very low endotoxin levels, making it ideal for transfection and other sensitive applications where endotoxins can be toxic to cells.
High Yield: The kit is optimized for large-scale plasmid preparation, typically isolating up to 1 mg of plasmid DNA from 500 mL to 1 liter of bacterial culture.
High Purity : The purified plasmid DNA is of excellent quality and free from RNA, proteins, and other contaminants, making it suitable for downstream applications such as sequencing, cloning, and transfection.
Efficient Removal of Contaminants : The kit uses a unique endotoxin removal step, which ensures that the plasmid DNA is purified with minimal contamination, particularly crucial for sensitive experiments like gene therapy.
Streamlined Process : The kit uses a modified alkaline lysis method combined with QIAGENs proprietary silica-based spin columns for a quick and easy protocol, making it faster and more efficient than traditional methods.
Components : Buffers : The kit includes specialized buffers for resuspension, lysis, neutralization, washing, and elution steps. The buffers are optimized to ensure high yields of endotoxin-free plasmid DNA.
EndoFree Chromatography Column : This column allows the binding, washing, and elution of plasmid DNA while removing endotoxins and other contaminants.
Elution Solution : A buffer or water to elute the purified, endotoxin-free plasmid DNA.
Benefits: Endotoxin-Free : Ideal for applications where endotoxins are problematic, such as cell transfection, gene therapy, and vaccine development.
High-Quality Plasmid DNA : Provides pure plasmid DNA, free of contaminants such as proteins and RNA, ensuring excellent performance in downstream applications.
Large-Scale Capacity : Suitable for large-scale plasmid DNA isolation, capable of handling bacterial cultures from 500 mL to 1 liter, producing high yields.
Simplified Workflow : The streamlined process reduces the number of steps, making it faster and less labor-intensive compared to traditional methods.
Versatility : Works with a variety of bacterial strains and plasmid sizes, making it a versatile tool for different research needs.

Applications : Gene Therapy : Ideal for preparing plasmid DNA for gene therapy, where the presence of endotoxins can hinder cellular uptake and expression.
Transfection : Ensures that the plasmid DNA is of high purity and free of contaminants, which is critical for successful transfection into mammalian cells.
Protein Production : Ideal for producing recombinant proteins in bacterial or eukaryotic expression systems.
Sequencing : Suitable for generating plasmid DNA for sequencing applications, ensuring high-quality, contaminant-free DNA for accurate results.
Vaccine Development : Provides endotoxin-free plasmid DNA for use in vaccine production or DNA immunization protocols.
The EndoFree Plasmid Maxi Kit (10) is an excellent choice for researchers who need high-purity, endotoxin-free plasmid DNA for sensitive applications.