Enterokinase Elisa Kit By Hangzhou Junfeng Bioengineering Co. Ltd
Get Latest Price
In Stock
Product Specifications
| Assay type | ELISA |
| Target | Enterokinase |
| Detection limit | 0.05ng/ml |
| Sample volume | 100ul |
| Plate format | 96-well |
| Incubation time | 60mins |
| Usage | Quantitatively detects Enterokinase in samples using a double antibody sandwich method. |
| Features | High sensitivity, Good specificity, Stable results, Quantitative assay, Easy to use |
Product Overview
Key Features
The kit uses anti Enterokinase antibody as the coating antibody, the enzyme labeled anti enterokinase antibody as the detection antibody, the highly sensitive TMB as the substrate, and the Enterokinase as the standard to quantitatively detect Enterokinase in the sample. This kit is characterized by good specificity, high sensitivity and stability,
The lowest detectable level of Enterokinase is 0.1ng/ml.
Principle
This kit uses double antibody sandwich method to detect Enterokinase residuals. The Enterokinase antibody is coated on the microplate, and the Enterokinase in the sample or standard is combined with the antibody fixed on the microplate. After the unconjugated sample or standard is washed with washing solution, the HRP-labeled Enterokinase antibody is added for incubation, and the enzyme labeled antibody is combined with Enterokinase. Wash the unconjugated enzyme labeled antibody, add TMB substrate solution to the microplate, incubate it in dark to form a blue complex, stop the reaction with stop solution, and the color changes from blue to yellow. Determine the absorbance value of the complex, and fit the standard curve with the OD value of the standard. The Enterokinase concentration of the sample can be calculated from the OD value of the sample through the formula fitted with the standard curve.
Kit components
ComponentsQTYPackage
Anti-Enterokinase antibody coated microtiter plate12 strips96T
Enterokinase STD5 vials1ml/vial
Enterokinase reference1 vial1ml/vial
Sample dilution buffer1 bottle10ml/bottle
HRP-labeled antibodyi 100Xi 1 vial100I l/vial
HRP-labeled antibody dilution buffer1 bottle10ml/bottle
Substrate solution1 bottle10ml/bottle
Stop solution1 bottle10ml/bottle
Wash buffer concentratei 20Xi 2 bottles25ml/bottle
COA1 copy
Manual1 copy
Specificity
Control absi NSBi <0.300
6.4ng/ml std abs >0.700
Precisioni %CV average<9.0%
Detection limit (>control 3SD) <0.05ng/ml
Storage and stability
Antibody-coated plate, HRP labeled antibody and Enterokinase standards are stored at -20a , other components are stored at 2-8a . It is stable for 6 months.
Catalog #
TE-19-003
The lowest detectable level of Enterokinase is 0.1ng/ml.
Principle
This kit uses double antibody sandwich method to detect Enterokinase residuals. The Enterokinase antibody is coated on the microplate, and the Enterokinase in the sample or standard is combined with the antibody fixed on the microplate. After the unconjugated sample or standard is washed with washing solution, the HRP-labeled Enterokinase antibody is added for incubation, and the enzyme labeled antibody is combined with Enterokinase. Wash the unconjugated enzyme labeled antibody, add TMB substrate solution to the microplate, incubate it in dark to form a blue complex, stop the reaction with stop solution, and the color changes from blue to yellow. Determine the absorbance value of the complex, and fit the standard curve with the OD value of the standard. The Enterokinase concentration of the sample can be calculated from the OD value of the sample through the formula fitted with the standard curve.
Kit components
ComponentsQTYPackage
Anti-Enterokinase antibody coated microtiter plate12 strips96T
Enterokinase STD5 vials1ml/vial
Enterokinase reference1 vial1ml/vial
Sample dilution buffer1 bottle10ml/bottle
HRP-labeled antibodyi 100Xi 1 vial100I l/vial
HRP-labeled antibody dilution buffer1 bottle10ml/bottle
Substrate solution1 bottle10ml/bottle
Stop solution1 bottle10ml/bottle
Wash buffer concentratei 20Xi 2 bottles25ml/bottle
COA1 copy
Manual1 copy
Specificity
Control absi NSBi <0.300
6.4ng/ml std abs >0.700
Precisioni %CV average<9.0%
Detection limit (>control 3SD) <0.05ng/ml
Storage and stability
Antibody-coated plate, HRP labeled antibody and Enterokinase standards are stored at -20a , other components are stored at 2-8a . It is stable for 6 months.
Catalog #
TE-19-003
Company Details
HANGZHOU JUNFENG BIOENGINEERING CO. LTD, Established in 2009 at Hangzhou in Zhejiang, is a leading Exporter,Manufacturer,Distributor,Supplier,Trading Company of Scientific Instruments in China. HANGZHOU JUNFENG BIOENGINEERING CO. LTD is one of Trade India's verified and trusted sellers of listed products. With extensive experience in supplying and trading Enterokinase ELISA Kit, HANGZHOU JUNFENG BIOENGINEERING CO. LTD has made a reputed name for itself in the market with high-quality BSA ELISA Kit, Benzonase ELISA Kit, Enterokinase ELISA Kit, etc.
Focusing on a customer-centric approach, HANGZHOU JUNFENG BIOENGINEERING CO. LTD has a pan-India presence and caters to a huge consumer base throughout the country. Buy Scientific Instruments in bulk from HANGZHOU JUNFENG BIOENGINEERING CO. LTD at Trade India quality-assured products.
Focusing on a customer-centric approach, HANGZHOU JUNFENG BIOENGINEERING CO. LTD has a pan-India presence and caters to a huge consumer base throughout the country. Buy Scientific Instruments in bulk from HANGZHOU JUNFENG BIOENGINEERING CO. LTD at Trade India quality-assured products.
Business Type
Exporter, Manufacturer, Distributor, Supplier, Trading Company
Employee Count
100
Establishment
2009
Working Days
Monday To Sunday
Related Products
Manual Trypsin Elisa Kit
MOQ - 10 Unit/Units
Hangzhou Junfeng Bioengineering Co. Ltd
Hangzhou, Zhejiang
TrypLE Trypsin ELISA Kit - Anti-TrypLEtrypsin Antibody Coated Microtiter Plate, 96 Tests , New Manual Operation for Medical Industry
MOQ - 10 Unit/Units
Hangzhou Junfeng Bioengineering Co. Ltd
Hangzhou, Zhejiang
HCV Antigen Rapid Test and ELISA Kit - Plastic, Double Antigen Sandwich Method | High Sensitivity, High Specificity, Rapid Accurate Results
Biomapper Technology Co,ltd
Ningbo, Zhejiang
Explore Related Categories
More Products From This Seller
Seller Details
TrustedSellerHangzhou, Zhejiang
Export Manager
Mr. Guoan Liu
Address
Liuxia Industry Park 11, North Liuhe Road, Xihu District, Hangzhou, Zhejiang, 310022, China
Report incorrect details
