Sumo Protease Elisa Kit - Double Antibody Sandwich, Detection Limit <0.5ng/ml | High Sensitivity, Good Specificity, Stable Results

The kit uses anti SUMO protease antibody as the coating antibody, the enzyme labeled anti SUMO protease antibody as the detection antibody, the highly sensitive TMB as the substrate, and the SUMO protease as the standard to quantitatively detect SUMO protease in the sample. This kit is characterized by good specificity, high sensitivity and stability,
The lowest detectable level of SUMO protease is 0.5ng/ml.
Principle
This kit uses double antibody sandwich method to detect SUMO protease residuals. The SUMO protease antibody is coated on the microplate, and the SUMO protease in the sample or standard is combined with the antibody fixed on the microplate. After the unconjugated sample or standard is washed with washing solution, the HRP-labeled SUMO protease antibody is added for incubation, and the enzyme labeled antibody is combined with SUMO protease. Wash the unconjugated enzyme labeled antibody, add TMB substrate solution to the microplate, incubate it in dark to form a blue complex, stop the reaction with stop solution, and the color changes from blue to yellow. Determine the absorbance value of the complex, and fit the standard curve with the OD value of the standard. The SUMO protease concentration of the sample can be calculated from the OD value of the sample through the formula fitted with the standard curve.
Kit components
ComponentsQTYPackage
Anti- SUMO protease antibody coated microtiter plate12 strips96T
SUMO protease STD1 vial100I l/vial
STD dilution buffer1 bottle10ml/bottle
HRP-labeled antibodyi 100Xi 1 vial100I l/vial
HRP-labeled antibody dilution buffer1 bottle10ml/bottle
Substrate solution1 bottle10ml/bottle
Stop solution1 bottle10ml/bottle
Wash buffer concentratei 20Xi 2 bottles25ml/bottle
COA1 copy
Manual1 copy
Specificity
Control absi NSBi <0.300
40ng/ml std abs >0.700
Precisioni %CV average<9.0%
Detection limit (>control 3SD) <0.5ng/ml
Storage and stability
Antibody-coated plate, HRP labeled antibody and SUMO protease standards are stored at -20a , other components are stored at 2-8a . It is stable for 6 months.