Bst Dna Polymerase

The Bst DNA polymerase is part of the DNA polymerase from Bacillus stearothermophilus expressed in E. coli, which is obtained through isolation and purification. The enzyme has 5a '-3' DNA polymerase activity, but does not have 5'- 3' exonuclease activity. Compared with other DNA polymerase, it has high thermal stability, chain replacement activity and polymerase activity, so it is more suitable for isothermal amplification, such as loop mediated isothermal PCR (LAMP), and 68a DNA sequencing (this condition is especially suitable for GC rich templates and nanogram level templates). This product cannot be used for thermal cycle sequencing or conventional PCR.
The enzyme activity is defined as the amount of enzyme required to mix 10 nmoL dNTP into acid insoluble sediment within 30 minutes at 65a .

Appearance: Colorless and clear liquid
Package: 2000U/Vial
Stocking buffer: 10 mM Tris-HCli pH 7.1
50 mM KCl
1 mM DTT
0.1 mM EDTA
50% Glycerol
Typical LAMP reaction
Add the following in 0.2ml thin wall reaction vial
Bst DNA polymerase2i -l
10A PCR buffer 5i -l
100 mM MgSO43AuL
10 mM dNTPs mix7AuL
F3 and B3 Primers (Final 200nM)x AuL
FIC and BIC Primers (Final 800nM) y AuL
Template DNA 1i 10i -l
Add water totally 50ul
Gently mix and centrifuge, then put it into a 65a water bath to react for 1 hour.

Stability: Stable at -20a for 36 months.