Stable Cell Line Construction Services - Antibody Yield 2.0-5.5 G/l, Cell Density 1.5e7/ml | High-yield Vector System, Suspension Culture, Animal-free

We are the leading service provider involved in providing services of Stable Cell Line Construction Services. To create stable cell lines is a key method in reducing the production costs of biopharmaceuticals. We use a modified FRT/Flp-In recombinase system to produce human proteins in CHO cells and 293 cells for stable expression. We have pre-selected CHO cell lines [and 293 cell lines] that contain a single stably integrated FRT site at a transcriptionally active genomic locus and we have proven that they can produce high level expressers of recombinant proteins.

We have also developed a unique series of high-yield vector system, in which two GC-rich regions flanking the gene expression cassette makes the promoter and enhancer continuously exposed to transcriptional machinery by preventing chromatin condensing. This revolutionary ground-breaking technology allows the available highest yields in all common mammalian cell protein production host cell lines, such as 293, CHO and NSO. Antibody yields of up to 2.0- 5.5 grams/liter [80-120pg/cell/day] have been achieved using this system in chemically defined, animal component-free medium. The selection strategy has been designed to identify cell lines that are highly productive and have the ability to grow well in suspension culture in a state-of-the-art, fed-batch fermentation process, in which cell density can reach 1.5A 107/mL.