Rat C5(complement Component 5) Elisa Kit

We Import, Distribute, Trade and Supply of Rat C5(Complement Component 5) ELISA Kit in Delhi, India. Intended use This ELISA kit applies to the in vitro quantitative determination of Rat C5 concentrations in serum, plasma and other biological fluids. Test principle This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat C5. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat C5 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat C5, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm A 2 nm. The OD value is proportional to the concentration of Rat C5. You can calculate the concentration of Rat C5 in the samples by comparing the OD of the samples to the standard curve. Assay type: Sandwich Format: 96T Assay time: 4.5h Reactivity: Rat Detection Method: Colormetric Detection Range: 0.31a 20 ng/mL Sensitivity: 0.19 ng/mL Sample Volume Required Per Well: 100 I L