Presto Mini Plasmid Kit

Our provided Presto Mini Plasmid Kit designed for plasmid and cosmid DNA purification from 1-7 ml of cultured bacterial cells. For processing larger volumes, the Prestoa c Midi Plasmid Kit is also available. Both plasmid kits include TrueBlue Lysis Buffer (an optional color indicator) to prevent common handling errors while ensuring efficient cell lysis, neutralization and maximum plasmid product yield. The Prestoa c Mini Plasmid Kit uses a modified alkaline lysis method and RNase treatment to obtain clear cell lysate with minimal genomic DNA/RNA contaminants. In the presence of chaotropic salt, plasmid DNA in the lysate binds to the glass fiber matrix of the plasmid spin column. Contaminants are removed with a Wash Buffer (containing ethanol) and the purified plasmid DNA is eluted by a low salt Elution Buffer, TE or water. Typical yields are 20-35 I g for high-copy number plasmid or 3-10 I g for low-copy number plasmid from 4 ml of cultured bacterial cells. DNA phenol extraction or alcohol precipitation are not required and the entire procedure can be completed within 15 minutes. Clients can avail this Presto Mini Plasmid Kit at industry leading prices from us.