Engreen Nuclease

Owing to our years of industry experience and market understanding, we are leading Exporter, Distributor & Supplier of Engreen Nuclease in Auckland, Auckland, New Zealand.
Application
Used for the removal of nucleic acid from protein samples.

Engreen nuclease, or endonuclease from Serratia marcescens, can be used to degrade all forms of DNA and RNA while having no proteolytic activity. Engreen nuclease can also be used to prepare proteins in microcalorimetric experiments.

The enzyme from Sigma has been used to limit cell clumping during the preparation of chimeric cell mixtures.[4] It has also been used for the preparation of nuclear extracts by digesting DNA and releasing nuclear proteins intimately associated with DNA.[7]

Biochem/physiol Actions

Digests native or heat-denatured DNA and RNA.

Benzonase is a genetically engineered endonuclease from Serratia marcescens.[12][11] The protein is a dimer of 30 kDa subunits with two essential disulfide bonds.[5][10][6][8] This endonuclease attacks and degrades all forms of DNA and RNA (single stranded, double stranded, linear and circular) and is effective over a wide range of operating conditions. The optimum pH for enzyme activity is found to be 8.0-9.2. It completely digests nucleic acids to 5a - monophosphate terminated oligonucleotides 3 to 5 bases in length. This is ideal for removal of nucleic acids from recombinant proteins and for applications where complete digestion of nucleic acids is desirable.