Chlorhexidine Gluconate Cas No: 5086-74-8

Certificate Of Analysis



Sr. No.TestSpecification
1DescriptionAlmost colorless or pale-yellowish liquid.
2Solubility

Miscible with water, with not more than 3 parts of acetone and with not more than 5 parts of ethanol (96 per cent).
3Identification
Infrared absorption


The IR spectrum of the substance being examined must be concordant with the IR spectrum obtained from Chlorhexidine CRS working standard.
Thin-layer chromatography


The principal spot in the chromatogram obtained with the test solution is similar in position, colour and size to the principal spot in the chromatogram obtained with the reference solution.
Melting rangeThe residue melts at 132oC to 136oC.
Chemical testA deep red colour is produced
4Specific gravityBetween 1.06 and 1.07
5pHBetween 5.5 and 7.0.
6Chloroaniline content

Any reddish-blue colour in the solution is not more intense than that in a standard prepared solution.
7Related substances by HPLC
8Total impuritiesNot more than 3.0%
9Assay by PotentiometricallyNot less than 19.0% and not more than 21.0 %.