Q. What is the temperature of autoclave machine?

Answer

By applying a constant pressure of around 15 psi to saturated steam, autoclaves can maintain a chamber temperature of at least 250 °F (121 °C) for a set period of time (often 30-60 minutes). Keeping air from becoming trapped is just as important as the right temperature and duration for attaining sterility. Sterilization calls for the use of steam and high temperatures on the material to be sterilized. This "wet heat" approach is widely recognized as the safest and most effective means of disinfecting potentially infectious trash and sterilizing laboratory implements. There is sufficient room for error when verifying decontamination methods because the spores can withstand 250°F for 5 minutes but are destroyed at that temperature in 13 minutes.