One-step Rt-pcr

We are engaged in manufacturing and exporting excellent quality One-Step RT-PCR to our valuable clients.
Size: 50 reactions

The One-Step RT-PCR system is designed for convenient, sensitive, and reproducible detection and analysis of RNA molecules by RT-PCR. Components for both cDNA synthesis and PCR are combined in a single tube, using gene-specific primers and target RNAs from either total RNA or mRNA. Reverse transcription automatically follows PCR cycling without additional steps. The system consists of two major components: RT/ HotStar Taq Mix and 2X Reaction Mix. The RT/ HotStar Taq Mix contains a mixture of Reverse Transcriptase and HotStar Taq DNA Polymerase for optimal cDNA synthesis and PCR amplification. The 2X Reaction Mix consists of a proprietary buffer system optimized for reverse transcription and PCR amplification, Mg2+ optimized for universal use,deoxyribonucleotide triphosphates, and stabilizers.

Components (50-rxn kit):
RT/ HotStar Taq Mix--50 I l
50 mM Magnesium Sulfate--200 I l
2X Reaction Mix (contain 0.4 mM of each dNTP, 6 mM MgSO4)--1.25 ml

Features:
Convenient and Sensitive Detection/Analysis of RNA Molecules by RT-PCR
Combines Reverse Transcriptase and HotStar TaqAa
Optimal cDNA Synthesis and PCR Amplification in one Single Reaction.